High-accuracy and video-rate lifetime extraction from time correlated single photon counting data on a graphical processing unit
نویسندگان
چکیده
منابع مشابه
Fluorescence lifetime imaging by time-correlated single-photon counting.
We present a time-correlated single photon counting (TCPSC) technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over the time of the fluorescence decay, the x-y coordinates of the scanning area, and the wavele...
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Time-resolved fluorescence spectroscopy is a powerful analysis tool in fundamental physics as well as in the life sciences. Implementing it in the time domain requires recording the time dependent intensity profile of the emitted light upon excitation by a short flash of light, typically a laser pulse. While in principle, one could attempt to record the time decay profile of the signal from a s...
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We describe a scanning time-of-flight system which uses the time-correlated single-photon counting technique to produce three-dimensional depth images of distant, noncooperative surfaces when these targets are illuminated by a kHz to MHz repetition rate pulsed laser source. The data for the scene are acquired using a scanning optical system and an individual single-photon detector. Depth images...
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The processing and analysis are described of range data in a time-of-flight imaging system based on time-correlated single photon counting. The system is capable of acquiring range data accurate to 1Opm at a standoff distance in the order of lm, although this can be varied substantially. It is shown how fitting of the pulsed histogram data by a combination of a symmetric key and polynomial func...
متن کاملFour-dimensional multiphoton microscopy with time-correlated single-photon counting.
We report on the implementation of fluorescence-lifetime imaging in multiphoton excitation microscopy that uses PC-compatible modules for time-correlated single-photon counting. Four-dimensional data stacks are produced with each pixel featuring fluorescence-decay curves that consist of as many as 4096 bins. Fluorescence lifetime(s) and their amplitude(s) are extracted by statistical methods at...
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ژورنال
عنوان ژورنال: Review of Scientific Instruments
سال: 2019
ISSN: 0034-6748,1089-7623
DOI: 10.1063/1.5115349